1JWK
Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457A Mutation at Tetrahydrobiopterin Binding Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-11-27 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 213.479, 213.479, 116.222 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.680 - 2.300 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nod |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.040 * | 0.380 * |
Total number of observations | 490274 * | |
Number of reflections | 63261 | |
<I/σ(I)> | 31.6 | 1.8 |
Completeness [%] | 91.4 | 59.7 |
Redundancy | 7.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 4 * | Ammonium sulfate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | EPPS | 40 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 27-30 (%sat) | |
4 | 1 | reservoir | MES | 50 (mM) | |
5 | 1 | reservoir | BOG | 10-30 (mM) | |
6 | 1 | reservoir | dithiothreitol | 15-30 (mM) |