1JVP
Crystal structure of human CDK2 (unphosphorylated) in complex with PKF049-365
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.528, 71.802, 72.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.530 |
| R-factor | 0.206 * |
| Rwork | 0.206 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.620 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.046 | 0.316 |
| Total number of observations | 179814 * | |
| Number of reflections | 39944 | |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 93.5 | 75.4 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 293 | PEG 4000, sodium chloride, ammonium acetate, sodium azide, Hepes, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.25 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 15 (mM) | pH7.4 | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.6 |
| 5 | 1 | reservoir | ammonium acetate | 50 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 10 (%) | |
| 7 | 1 | reservoir | sodium azide | 0.02 (%) |
