1JVA
CRYSTAL STRUCTURE OF THE VMA1-DERIVED ENDONUCLEASE BEARING THE N AND C EXTEIN PROPEPTIDES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 0.7000 |
Spacegroup name | P 1 |
Unit cell lengths | 66.178, 68.846, 58.524 |
Unit cell angles | 103.10, 98.79, 78.98 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vde |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.032 | 0.155 |
Total number of observations | 122186 * | |
Number of reflections | 51056 | 4038 * |
<I/σ(I)> | 30.6 | 2.63 |
Completeness [%] | 89.4 | 70.9 |
Redundancy | 2.39 | 1.63 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 20 * | PEG6000, BisTrisHCl, mercaptoethanol, magnesium chloride, cadmium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG6000 | 17 (%(w/v)) | |
2 | 1 | reservoir | Bis-Tris-HCl | 0.1 (M) | pH6.2 |
3 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | 1 (mM) |