1JV4

Crystal structure of recombinant major mouse urinary protein (rmup) at 1.75 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1998-10-30
Detector	MARRESEARCH
Wavelength(s)	1.39
Spacegroup name	P 1 21 1
Unit cell lengths	37.140, 55.790, 37.670
Unit cell angles	90.00, 93.24, 90.00

Refinement procedure

Resolution	14.000 - 1.750
R-factor	0.206 *
Rwork	0.206
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mup.pdb
RMSD bond length	0.028 *
RMSD bond angle	2.940 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.000	1.760
High resolution limit [Å]	1.750 *	1.700
Rmerge	0.075 *	0.299 *
Number of reflections	38851
<I/σ(I)>	10.9	2.46
Completeness [%]	86.4 *	89.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	60mM CdCl2 and sodium phosphate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		60 (mM)
2	1	drop	protein	10 (mg/ml)