1JUV
Crystal structure analysis of Dihydrofolate reductase from Bacteriophage T4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 2000-01-01 |
Detector | WEISSENBERG |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 61.160, 61.160, 124.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.570 - 1.700 |
R-factor | 0.248 |
Rwork | 0.227 |
R-free | 0.26900 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.068 | |
Number of reflections | 26714 | |
Completeness [%] | 99.7 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 295 | NaCl, (NH4)2HPO4 , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |