1JTT
Degenerate interfaces in antigen-antibody complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.220, 72.840, 38.660 |
| Unit cell angles | 90.00, 106.94, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.100 |
| R-factor | 0.178 |
| Rwork | 0.164 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mel |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.760 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.150 | 0.432 |
| Number of reflections | 19935 | |
| <I/σ(I)> | 11.5 | 3.48 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | 2M NaFormate, 100mM Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
