1JTP
Degenerate interfaces in antigen-antibody complexes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-08-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98089 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.123, 69.375, 113.231 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.000 - 1.900 |
Rwork | 0.184 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code: 1MEL |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.061 | 0.417 |
Number of reflections | 40619 | |
<I/σ(I)> | 11.4 | 2 |
Completeness [%] | 95.7 | 92 |
Redundancy | 2.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | 2M Na Formate, 100mM Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |