1JT5

Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form with Amino Terminal His Tag AND LEU 73 REPLACED BY VAL AND VAL 109 REPLACED BY LEU (L73V/V109L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2000-11-28
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	74.163, 97.923, 108.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.900 - 1.850
R-factor	0.199 *
R-free	0.23600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jqz
RMSD bond length	0.007
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MRCHK
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.900	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.074	0.415
Total number of observations	250738 *
Number of reflections	32924 *
<I/σ(I)>	22.8	3.3
Completeness [%]	99.5	92.8
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 or 10 *	sodium formate, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9-12 (mg/ml)
2	1	reservoir	sodium formate	3.4-4.4 (M)
3	1	reservoir	ammonium sulfate	1.0-1.2 (M)