1JSY
Crystal structure of bovine arrestin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.948 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.952, 78.952, 158.924 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 2.900 |
| R-factor | 0.2298 * |
| Rwork | 0.230 |
| R-free | 0.23850 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CF1; CHAIN B |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.580 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.074 * | 0.318 * |
| Number of reflections | 13476 | |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 99.9 * | 99.8 |
| Redundancy | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | magnesium formate, polypropylene glycol 400, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 5 (mM) | pH7.2 |
| 3 | 1 | drop | EDTA | 0.5 (mM) | |
| 4 | 1 | drop | 62.5 (mM) | ||
| 5 | 1 | drop | magnesium formate | 75 (mM) | |
| 6 | 1 | drop | PEG400 | 20 (%) | |
| 7 | 1 | reservoir | magnesium formate | 150 (mM) |
