1JS0

Crystal Structure of 3D Domain-swapped RNase A Minor Trimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.072
Spacegroup name	P 21 21 2
Unit cell lengths	114.590, 122.170, 26.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.184 *
Rwork	0.184
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtb
RMSD bond length	0.014
RMSD bond angle	1.906 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.096 *
Total number of observations	327545 *
Number of reflections	22989
<I/σ(I)>	18.6	4.4
Completeness [%]	99.7	99.8
Redundancy	14	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7 *	4 *	PEG 10,000, amonium sulfate, pH 3.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	phosphate	0.18 (M)	pH6.7