1JR2
Structure of Uroporphyrinogen III Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-06-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 |
Unit cell lengths | 39.852, 59.251, 61.944 |
Unit cell angles | 80.47, 73.32, 88.33 |
Refinement procedure
Resolution | 58.720 - 1.840 |
R-factor | 0.2031 |
Rwork | 0.200 |
R-free | 0.25099 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.045 * | 0.327 * |
Total number of observations | 201469 * | |
Number of reflections | 43540 | |
<I/σ(I)> | 22 | 2.6 |
Completeness [%] | 94.0 | 75.8 * |
Redundancy | 3 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 * | 4 * | MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | MPD | 20 (%) | |
3 | 1 | reservoir | MES | 100 (mM) |