1JPY

Crystal structure of IL-17F

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790
Spacegroup name	P 65
Unit cell lengths	126.438, 126.438, 89.914
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.850
R-factor	0.238
Rwork	0.238
R-free	0.28400
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.600
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (98.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.088	0.541
Total number of observations	141778 *
Number of reflections	19294
<I/σ(I)>	7.7
Completeness [%]	100.0	100
Redundancy	7.3	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	19 *	well solution consisted of 1.0M Lithium Sulfate, 0.5M Ammonium Sulfate, 100mM Sodium Citrate, 1% Ethanol at 5.6, VAPOR DIFFUSION, HANGING DROP at 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	lithium sulfate	1.0 (M)
2	1	reservoir	ammonium sulfate	0.5 (M)
3	1	reservoir	ethanol	1 (%)
4	1	reservoir	sodium citrate	100 (mM)