1JPM

L-Ala-D/L-Glu Epimerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	113
Detector technology	CCD
Collection date	2000-06-01
Detector	CUSTOM-MADE
Wavelength(s)	0.95372
Spacegroup name	I 4
Unit cell lengths	157.630, 157.630, 168.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.250
R-factor	0.197
Rwork	0.197
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.072	0.416
Total number of observations	570691 *
Number of reflections	97014
<I/σ(I)>	12.3	4.1
Completeness [%]	99.9 *	99.9
Redundancy	5.9	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	293	8% Peg400, 0.2M MgCl2,50 mM BisTRIS, pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	8 (%)
2	1	reservoir		0.2 (M)
3	1	reservoir	Bis-Tris	50 (mM)	pH5.0