1JPA

Crystal Structure of unphosphorylated EphB2 receptor tyrosine kinase and juxtamembrane region

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Detector technology	CCD
Collection date	2000-05-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790, 0.9788, 0.9770
Spacegroup name	P 1
Unit cell lengths	47.052, 57.616, 67.742
Unit cell angles	112.95, 103.17, 91.58

Refinement procedure

Resolution	25.650 - 1.910
R-factor	0.231 *
Rwork	0.231
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	apo-form from MAD data
RMSD bond length	0.007
RMSD bond angle	21.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *
Rmerge	0.033 *	0.146 *
Total number of observations	80035 *
Number of reflections	43865 *
Completeness [%]	90.3 *	55.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	301	HEPES, magnesium chloride, PEG 4000, isopropanol, ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 301K
1	VAPOR DIFFUSION, HANGING DROP	7	301	HEPES, magnesium chloride, PEG 4000, isopropanol, ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 301K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.5 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir		0.2 (M)
4	1	reservoir	PEG4000	10 (%(w/v))
5	1	reservoir	isopropanol	10 (%(v/v))
6	1	reservoir	ethylene glycol	15 (%(v/v))