1JLU
Crystal Structure of the Catalytic Subunit of cAMP-dependent Protein Kinase Complexed with a Phosphorylated Substrate Peptide and Detergent
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-07-01 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.870, 75.580, 80.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1atp |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.400 |
| Data reduction software | UCSD-system |
| Data scaling software | UCSD-system |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.043 * | |
| Number of reflections | 19924 | |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Zheng, J., (1992) Acta Crystallogr., B48, 241. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium acetate | 150 (mM) | |
| 3 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 6 | 1 | reservoir | PEG400 | 8 (%(w/v)) | |
| 7 | 1 | reservoir | methanol | 15 (%(v/v)) | |
| 8 | 1 | reservoir | detergent MEGA-8 | 0.4 (%(w/v)) |
