1JLS
STRUCTURE OF THE URACIL PHOSPHORIBOSYLTRANSFERASE URACIL/CPR 2 MUTANT C128V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-04-23 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.400, 111.400, 71.900 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 60.000 * - 2.500 |
| R-factor | 0.225 * |
| Rwork | 0.232 |
| R-free | 0.26500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bd3 |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 2.180 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | MERLOT |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.101 | 0.265 |
| Total number of observations | 99823 * | |
| Number of reflections | 32692 | |
| <I/σ(I)> | 13.5 | 1.5 |
| Completeness [%] | 84.6 | 56 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 * | 298 | NaCl, citrate/phosphate buffer, PEG 3400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | reservoir | sodium citrate/phosphate | 15 (mM) | pH4.7 |
| 3 | 1 | reservoir | PEG3400 | 10 (%) |
