1JLN

Crystal structure of the catalytic domain of protein tyrosine phosphatase PTP-SL/BR7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1999-01-26
Detector	SIEMENS HI-STAR
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.136, 65.322, 105.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.690 - 1.810
R-factor	0.195
Rwork	0.195
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yfo
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	X-GEN
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.690	1.900
High resolution limit [Å]	1.810	1.810
Rmerge	0.057
Total number of observations	118144 *
Number of reflections	24970
<I/σ(I)>	17.1	2.62
Completeness [%]	83.7	40.3
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	6 (%)
2	1	reservoir	Tris-HCl	100 (mM)