1JL9
Crystal Structure of Human Epidermal Growth Factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Synchrotron site | APS |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 61.430, 61.430, 87.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 3.000 |
R-factor | 0.231 * |
Rwork | 0.231 |
R-free | 0.28300 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.193 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.104 | |
Total number of observations | 21097 * | |
Number of reflections | 4040 | |
Completeness [%] | 99.0 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 291 * | magnesium chloride, Bicine, CYMAL-3, sodium azide, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 0.9 (M) | ||
2 | 1 | reservoir | CYMAL-3 | 3.5 (mM) | |
3 | 1 | reservoir | Bicine | 0.1 (M) | |
4 | 1 | drop | protein | 50 (mg/ml) |