1JL4
CRYSTAL STRUCTURE OF THE HUMAN CD4 N-TERMINAL TWO DOMAIN FRAGMENT COMPLEXED TO A CLASS II MHC MOLECULE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-12 |
| Detector | SBC-2 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 145.195, 145.195, 103.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.300 |
| R-factor | 0.428 |
| Rwork | 0.420 |
| R-free | 0.45300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1IAK and 3CD4 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.500 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Rmerge | 0.152 | 0.610 |
| Number of reflections | 7428 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 83.0 | 56 |
| Redundancy | 9.6 * | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297 | 17% PEG 4,000/0.2M Li2SO4/0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 17 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | Tris | 0.1 (M) |
