1JKU
Crystal Structure of Manganese Catalase from Lactobacillus plantarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.720, 96.420, 106.690 |
Unit cell angles | 90.00, 106.88, 90.00 |
Refinement procedure
Resolution | 14.900 * - 1.840 |
R-factor | 0.14691 |
Rwork | 0.145 |
R-free | 0.20000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | hexamer of Thermus thermophilus Mn catalase |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 1.886 |
High resolution limit [Å] | 1.840 | 1.840 * |
Rmerge | 0.055 * | 0.136 |
Total number of observations | 157987 * | |
Number of reflections | 113270 * | |
<I/σ(I)> | 3 | |
Completeness [%] | 90.4 * | 63.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 * | 17 * | PEG 8000, TAPS, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 25 (%) | |
3 | 1 | reservoir | TAPS | 0.1 (M) | pH8.7 |