1JKU

Crystal Structure of Manganese Catalase from Lactobacillus plantarum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-06-12
Detector	MARRESEARCH
Wavelength(s)	0.8900
Spacegroup name	P 1 21 1
Unit cell lengths	74.720, 96.420, 106.690
Unit cell angles	90.00, 106.88, 90.00

Refinement procedure

Resolution	14.900 * - 1.840
R-factor	0.14691
Rwork	0.145
R-free	0.20000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	hexamer of Thermus thermophilus Mn catalase
RMSD bond length	0.012 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	1.886
High resolution limit [Å]	1.840	1.840 *
Rmerge	0.055 *	0.136
Total number of observations	157987 *
Number of reflections	113270 *
<I/σ(I)>		3
Completeness [%]	90.4 *	63.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7 *	17 *	PEG 8000, TAPS, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG8000	25 (%)
3	1	reservoir	TAPS	0.1 (M)	pH8.7