1JH6
Semi-reduced Cyclic Nucleotide Phosphodiesterase from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 99.380, 99.380, 175.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.000 * - 1.800 |
R-factor | 0.192 * |
Rwork | 0.192 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | oxidised CPDase (A. thaliana) [delta 90-115] |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.075 | 0.474 |
Total number of observations | 306271 * | |
Number of reflections | 29387 | |
<I/σ(I)> | 19 | |
Completeness [%] | 94.1 | 95.7 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 285 | (NH4)2SO4, NaOAc, DTT, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.2-2.0 (M) | |
2 | 1 | reservoir | 0.1 (M) | pH5.0 | |
3 | 1 | drop | DTT | 12.5 (mM) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
6 | 1 | drop | protein | 10 (mg/ml) |