1JG5

CRYSTAL STRUCTURE OF RAT GTP CYCLOHYDROLASE I FEEDBACK REGULATORY PROTEIN, GFRP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	106.803, 110.052, 38.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.166 *
Rwork	0.166
R-free	0.21400
Structure solution method	MIR
RMSD bond length	0.007 *
RMSD bond angle	1.380 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PROTEIN
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.650
High resolution limit [Å]	2.600	2.600
Rmerge	0.118
Total number of observations	154398 *
Number of reflections	14791
Completeness [%]	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.4	4 *	100mM potassium phosphate, 2mM DTT, pH 7.4, batch crystallization, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	potassium phosphate	100 (mM)
2	1	1	dithiothreitol	2 (mM)
3	1	1	protein	5 (mg/ml)