1JG5
CRYSTAL STRUCTURE OF RAT GTP CYCLOHYDROLASE I FEEDBACK REGULATORY PROTEIN, GFRP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 106.803, 110.052, 38.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.166 * |
Rwork | 0.166 |
R-free | 0.21400 |
Structure solution method | MIR |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.380 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PROTEIN |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.118 | |
Total number of observations | 154398 * | |
Number of reflections | 14791 | |
Completeness [%] | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.4 | 4 * | 100mM potassium phosphate, 2mM DTT, pH 7.4, batch crystallization, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | potassium phosphate | 100 (mM) | |
2 | 1 | 1 | dithiothreitol | 2 (mM) | |
3 | 1 | 1 | protein | 5 (mg/ml) |