1JEY
Crystal Structure of the Ku heterodimer bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.920, 126.305, 126.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.500 |
R-factor | 0.218 * |
Rwork | 0.218 |
R-free | 0.27600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jeq |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.220 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.065 | 0.311 * |
Total number of observations | 141891 * | |
Number of reflections | 46385 * | |
<I/σ(I)> | 17.5 | 3.6 |
Completeness [%] | 96.4 | 82.3 * |
Redundancy | 3.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 22 * | 26% polyethylene glycol 350 monomethyl ether, 50 mM Tris-HCL pH 8.0, 5 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 350 monomethyl ether | ||
2 | 1 | 1 | Tris-HCl | ||
3 | 1 | 1 | MgCl2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | reservoir | PEG350MME | 26 (%) | |
3 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
4 | 1 | reservoir | 5 (mM) |