1JEY
Crystal Structure of the Ku heterodimer bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.920, 126.305, 126.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.500 |
| R-factor | 0.218 * |
| Rwork | 0.218 |
| R-free | 0.27600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jeq |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.220 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.065 | 0.311 * |
| Total number of observations | 141891 * | |
| Number of reflections | 46385 * | |
| <I/σ(I)> | 17.5 | 3.6 |
| Completeness [%] | 96.4 | 82.3 * |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 22 * | 26% polyethylene glycol 350 monomethyl ether, 50 mM Tris-HCL pH 8.0, 5 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 350 monomethyl ether | ||
| 2 | 1 | 1 | Tris-HCl | ||
| 3 | 1 | 1 | MgCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | reservoir | PEG350MME | 26 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
| 4 | 1 | reservoir | 5 (mM) |
