1JEB
Chimeric Human/Mouse Carbonmonoxy Hemoglobin (Human Zeta2 / Mouse Beta2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 |
| Unit cell lengths | 84.921, 84.921, 104.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 * - 2.100 |
| Rwork | 0.216 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bbb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.053 | 0.465 |
| Total number of observations | 192738 * | |
| Number of reflections | 43348 | |
| <I/σ(I)> | 11.5 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 18 * | Bicine/NaOH, MePEG 550, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | HEPES | 0.1 (M) | pH7.4 |
| 3 | 1 | drop | sodium dithionite | 2 (mM) | |
| 4 | 1 | reservoir | Bicine-NaOH | 0.1 (M) | pH8.5 |
| 5 | 1 | reservoir | MePEG550 | 33 (%) |
