1JD2
Crystal Structure of the yeast 20S Proteasome:TMC-95A complex: A non-covalent Proteasome Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.050 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.200, 301.300, 144.800 |
| Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.271 |
| Rwork | 0.251 |
| R-free | 0.33600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.987 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.124 |
| Total number of observations | 741075 * |
| Number of reflections | 255383 * |
| <I/σ(I)> | 4.22 |
| Completeness [%] | 98.4 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 24 * | MPD, MES, DMSO, magnesium Acetate, pH 6.4, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | magnesium acetate | 30 (mM) | |
| 5 | 1 | reservoir | MES | 100 (mM) | pH7.2 |
| 6 | 1 | reservoir | MPD | 10 (%(v/v)) |
