1JCZ
CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF HUMAN CARBONIC ANHYDRASE XII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 97 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.733, 44.578, 85.167 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0e |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.046 | 0.287 |
| Total number of observations | 402312 * | |
| Number of reflections | 80168 * | |
| <I/σ(I)> | 17.3 | 4.1 |
| Completeness [%] | 96.5 | 79.3 |
| Redundancy | 5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | PEG 8000, sodium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris sulfate | 10 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 3-5 (%(w/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) |
