1JBM
Heptameric crystal structure of Mth649, an Sm-like archaeal protein from Methanobacterium thermautotrophicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-03-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.10000 |
Spacegroup name | P 1 |
Unit cell lengths | 45.066, 54.077, 62.346 |
Unit cell angles | 87.58, 72.86, 81.45 |
Refinement procedure
Resolution | 20.000 - 1.850 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i8f |
RMSD bond length | 0.021 |
RMSD bond angle | 2.060 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.054 | 0.255 |
Number of reflections | 44472 | |
<I/σ(I)> | 20.4 | 4.14 |
Completeness [%] | 93.8 | 67.4 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | PEG-4000, acetate, citrate buffer, NaCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |