1JBM
Heptameric crystal structure of Mth649, an Sm-like archaeal protein from Methanobacterium thermautotrophicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.066, 54.077, 62.346 |
| Unit cell angles | 87.58, 72.86, 81.45 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i8f |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.060 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.054 | 0.255 |
| Number of reflections | 44472 | |
| <I/σ(I)> | 20.4 | 4.14 |
| Completeness [%] | 93.8 | 67.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | PEG-4000, acetate, citrate buffer, NaCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
