1JBB
Ubiquitin Conjugating Enzyme, Ubc13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.960, 129.390, 42.920 |
| Unit cell angles | 90.00, 96.42, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.188 * |
| Rwork | 0.183 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QCQ truncated to ALAs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.223 |
| Total number of observations | 55421 * | |
| Number of reflections | 17787 | |
| <I/σ(I)> | 27.8 | 4.4 |
| Completeness [%] | 93.6 | 83.2 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG 5000-MME, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | PEG5000 MME | 30 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 7 | 1 | reservoir | MES | 50 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 2 (mM) |
