1J95
KCSA potassium channel with TBA (tetrabutylammonium) and potassium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.900, 67.400, 112.000 |
| Unit cell angles | 90.00, 125.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.32 |
| Rwork | 0.298 |
| R-free | 0.32200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bl8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.265 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | 0.458 |
| Number of reflections | 12054 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 92.6 | 93.1 |
| Redundancy | 2.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20 * | PEG 400, HEPES, POTASSIUM CHLORIDE, CALCIUM CHLORIDE, DETERGENT LDAO, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris | 50 (mM) | |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | 200 (mM) | ||
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | PEG400 | 48 (%) |
