1J8E

Crystal structure of ligand-binding repeat CR7 from LRP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	200
Detector technology	CCD
Collection date	2001-02-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	27.588, 35.359, 36.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.960 - 1.850
R-factor	0.186 *
Rwork	0.186
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Composite search model constructed based on coordinates of 1AJJ 1d2l and 1CR8.
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.970
High resolution limit [Å]	1.850	1.850
Rmerge	0.072	0.268
Total number of observations	86028 *
Number of reflections	2911 *
<I/σ(I)>	20
Completeness [%]	88.5	56.6
Redundancy	5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	18 *	0.02M Na-acetate, 0.1M CaCl2, 0.3M NaCl, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir		0.3 (M)
3	1	reservoir	sodium acetate	20 (mM)	pH3.8
4	1	reservoir		100 (mM)