1J53
Structure of the N-terminal Exonuclease Domain of the Epsilon Subunit of E.coli DNA Polymerase III at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.800, 60.800, 111.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.199 |
| R-free | 0.22700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.265 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.041 | 0.204 |
| Number of reflections | 20091 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | Hamdan, S., (2000) J.Struct.Biol., 131, 164. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium HEPES | 50 (mM) | pH7.5 |
| 2 | 1 | drop | dithiothreitol | 2 (mM) | |
| 3 | 1 | drop | protein | 11.5 (mg/ml) | |
| 4 | 1 | drop | TMP | 5 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 20-21 (%) | |
| 7 | 1 | reservoir | cacodylate | 0.1 (M) | pH5.8 |
