Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.390, 55.770, 101.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.750 |
| R-factor | 0.188 * |
| Rwork | 0.188 |
| R-free | 0.26400 * |
| Structure solution method | MR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MERLOT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.095 | 0.290 |
| Total number of observations | 16753 * | |
| Number of reflections | 9234 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 90.0 | 80 |
| Redundancy | 1.5 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 * | 298 | PEG 4000, ISOPROPANOL, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-15 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | 2-propanol | 10 (%) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) | pH5.6 |
