1J35
Crystal Structure of Ca(II)-bound Gla Domain of Factor IX Complexed with Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.72 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.959, 39.074, 63.949 |
| Unit cell angles | 90.00, 99.56, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iod |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.046 | 0.252 |
| Number of reflections | 27532 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 94.0 * | 72.4 |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 20 * | PEG 6000, Tris-HCl, calcium chloride, pH 8, MICROBATCH, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5 (mg/ml) | |
| 2 | 1 | 2 | Tris-HCl | 30 (mM) | pH8.0 |
| 3 | 1 | 2 | PEG6000 | 14 (%) | |
| 4 | 1 | 2 | 5 (mM) | ||
| 5 | 1 | 2 | 2 (mM) |
