1J2G
Crystal structure of Urate oxidase from Bacillus SP. TB-90 co-crystallized with 8-Azaxanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.900 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 133.390, 144.410, 78.785 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.010 - 2.200 |
R-factor | 0.18232 |
Rwork | 0.180 |
R-free | 0.21806 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.503 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.010 | 2.257 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.066 | 0.066 |
Number of reflections | 75560 | |
<I/σ(I)> | 7.9 | 2.1 |
Completeness [%] | 97.0 | 90 |
Redundancy | 6 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, lithium sulfate, Tris-HCl, 8-azaxanthine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |