1J2F

X-ray crystal structure of IRF-3 and its functional implications

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-25
Detector	OXFORD PX210
Wavelength(s)	0.9
Spacegroup name	P 4 21 2
Unit cell lengths	134.808, 134.808, 69.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 2.300
R-factor	0.2194
Rwork	0.217
R-free	0.24200 *
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.260 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.017	2.110
High resolution limit [Å]	2.300 *	1.999
Rmerge	0.094 *
Number of reflections	24036 *
Completeness [%]	94.6 *	95.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9 *	4 *	magnesium formate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	50 (mM)	pH7.9
3	1	drop		500 (mM)
4	1	reservoir	sodium acetate	100 (mM)	pH5.1
5	1	reservoir	magnesium formate	400 (mM)