1J25
Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain, Mn cocrystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.795, 69.795, 58.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.780 |
| Rwork | 0.228 |
| R-free | 0.25200 |
| Structure solution method | MAD |
| Starting model (for MR) | selenomet-phased model |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.090 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.780 | 1.790 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 15973 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.2 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 8 | 20 * | Tricine-NaOH, NaCl, NaN3, subtilisin, MnCl2 , pH 8.0, MICRODIALYSIS, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 60 (mg/ml) | |
| 2 | 1 | 2 | tricine-NaOH | 100 (mM) | pH8.0 |
| 3 | 1 | 2 | 300 (mM) | ||
| 4 | 1 | 2 | 0.025 (%) |
