1J1J
Crystal Structure of human Translin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.836 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 129.420, 135.270, 134.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.128 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 * | 0.414 * |
| Number of reflections | 57689 * | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 97.3 | 97.3 |
| Redundancy | 5.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 293 | Sodium Formate, Sodium Chloride, Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 5 | 1 | reservoir | sodium formate | 2.5 (M) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.5 |
