1J1J

Crystal Structure of human Translin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.836
Spacegroup name	C 2 2 21
Unit cell lengths	129.420, 135.270, 134.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.231
Rwork	0.229
R-free	0.26500
Structure solution method	MAD
RMSD bond length	0.007 *
RMSD bond angle	1.128 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.087 *	0.414 *
Number of reflections	57689 *
<I/σ(I)>		1.8
Completeness [%]	97.3	97.3
Redundancy	5.1	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	293	Sodium Formate, Sodium Chloride, Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.0
3	1	drop		150 (mM)
4	1	drop	glycerol	5 (%(v/v))
5	1	reservoir	sodium formate	2.5 (M)
6	1	reservoir	sodium acetate	100 (mM)	pH4.5