1J1D
Crystal structure of the 46kDa domain of human cardiac troponin in the Ca2+ saturated form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-12-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.346, 167.860, 69.707 |
| Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.610 |
| Rwork | 0.264 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 18.750 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.042 | 0.223 |
| Total number of observations | 103050 * | |
| Number of reflections | 27912 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 96.7 | 84.6 |
| Redundancy | 3.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, lithium chloride, Tris-HCl, calcium chloride, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 20 (%) | |
| 2 | 1 | reservoir | glycerol | 15 (%) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 5 | 1 | reservoir | 5 (mM) | or SrCl2, pH8. | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
