1J0O
High Resolution Crystal Structure of the wild type Tetraheme Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.71 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.336, 67.347, 34.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.150 |
| R-factor | 0.1042 |
| Rwork | 0.101 |
| R-free | 0.16690 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.044 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.299 | 1.210 |
| High resolution limit [Å] | 1.150 * | 1.150 |
| Rmerge | 0.059 | 0.143 |
| Number of reflections | 40815 | |
| Completeness [%] | 93.5 | 56.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 | 10 * | ethanol, pH 7.4, SMALL TUBES, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 12.5 (mM) | pH7.4 |
| 3 | 1 | drop | ethanol | 50 (%(v/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 10 (mM) | pH7.4 |
| 5 | 1 | reservoir | ethanol | 60 (%(v/v)) |
