1J0O

High Resolution Crystal Structure of the wild type Tetraheme Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-13
Detector	MARRESEARCH
Wavelength(s)	0.71
Spacegroup name	P 21 21 21
Unit cell lengths	52.336, 67.347, 34.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.150
R-factor	0.1042
Rwork	0.101
R-free	0.16690
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.022
RMSD bond angle	0.044
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.299	1.210
High resolution limit [Å]	1.150 *	1.150
Rmerge	0.059	0.143
Number of reflections	40815
Completeness [%]	93.5	56.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4	10 *	ethanol, pH 7.4, SMALL TUBES, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl	12.5 (mM)	pH7.4
3	1	drop	ethanol	50 (%(v/v))
4	1	reservoir	Tris-HCl	10 (mM)	pH7.4
5	1	reservoir	ethanol	60 (%(v/v))