1J09
Crystal structure of Thermus thermophilus glutamyl-tRNA synthetase complexed with ATP and Glu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.920, 82.620, 83.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1GLN |
| RMSD bond length | 0.005 |
| RMSD bond angle | 21.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.444 |
| Total number of observations | 208385 * | |
| Number of reflections | 49026 | |
| Completeness [%] | 91.7 | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 4 or 20 * | PEG 6000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.0 (mg/ml) | |
| 2 | 1 | drop | MOPS/Na | 10 (mM) | pH6.5 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 2.5 (mM) | |
| 5 | 1 | drop | PEG6000 | 1 (%) | |
| 6 | 1 | drop | ATP | 2 (mM) | |
| 7 | 1 | drop | glutamate | 2 (mM) | |
| 8 | 1 | reservoir | PEG | 10 (%) |
