1J04
Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.491, 89.491, 142.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.600 |
| Rwork | 0.223 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.092 | 0.487 |
| Number of reflections | 18539 | |
| <I/σ(I)> | 11.6 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | PEG 4000, Na HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
