1IZN
Crystal Structure of Actin Filament Capping Protein CapZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-24 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.200, 57.200, 99.200 |
| Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.222 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.156 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.051 | 0.265 |
| Total number of observations | 228951 * | |
| Number of reflections | 61088 | |
| <I/σ(I)> | 26 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG3350, magnesium nitrate, MES-NAOH, Jeffamine M-600, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | Jeffamine M-600 | 5 (%) | |
| 5 | 1 | reservoir | PEG3350 | 18 (%) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | MES-NaOH | 0.1 (M) | pH6.0 |
