1IYJ
STRUCTURE OF A BRCA2-DSS1 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2001-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 43 |
| Unit cell lengths | 130.314, 130.314, 192.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.400 |
| R-factor | 0.248 |
| Rwork | 0.244 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.550 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.520 |
| High resolution limit [Å] | 3.100 | 3.400 |
| Number of reflections | 41234 | |
| Completeness [%] | 98.2 | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | Li2SO4, NH4OCOCH3, Bis-tris Propane, DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
