1IVL
THE DE NOVO DESIGN OF AN ANTIBODY COMBINING SITE: CRYSTALLOGRAPHIC ANALYSIS OF THE VL DOMAIN CONFIRMS THE STRUCTURAL MODEL
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.890, 58.050, 83.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.170 |
R-factor | 0.175 |
Rwork | 0.175 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.700 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 * |
High resolution limit [Å] | 2.170 * |
Rmerge | 0.060 * |
Total number of observations | 69140 * |
Number of reflections | 12009 * |
Completeness [%] | 91.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.75 * | 16-22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | isopropanol | 20 (%(v/v)) | |
3 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | |
5 | 1 | reservoir | EDTA | 0.1 (mM) | |
6 | 1 | reservoir | sodium azide | 0.01 (%(w/v)) |