1IVH
STRUCTURE OF HUMAN ISOVALERYL-COA DEHYDROGENASE AT 2.6 ANGSTROMS RESOLUTION: STRUCTURAL BASIS FOR SUBSTRATE SPECIFICITY
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-04 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.000, 97.700, 181.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1MDE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.530 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.219 * |
| Number of reflections | 42529 | |
| <I/σ(I)> | 11.5 | 2.3 |
| Completeness [%] | 82.2 | 61 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 19 * | PROTEIN WAS CRYSTALLIZED FROM 8% PEG 8000, 0.1 M TRIS, PH 8.5 AT 19 DEGREES CELSIUS., temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | human IVD | 8 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 4 (%(w/v)) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 8 (%(w/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) |
