1ITQ

HUMAN RENAL DIPEPTIDASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-05-22
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	81.948, 81.213, 57.164
Unit cell angles	90.00, 96.73, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.187
Rwork	0.181
R-free	0.25800
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	23.190 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.093	0.163
Total number of observations	174691 *
Number of reflections	30657
<I/σ(I)>	11
Completeness [%]	92.3	79.5
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	10 *	Nitanai, Y., (1996) J.CRYST.GROWTH, 168, 280. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG8000	7.5 (%(w/v))	pH5.6
3	1	reservoir	PEG8000	15 (%)	pH5.6