1IS8
Crystal structure of rat GTPCHI/GFRP stimulatory complex plus Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2818 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.900, 111.430, 125.910 |
| Unit cell angles | 90.00, 97.32, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.216 * |
| Rwork | 0.219 |
| R-free | 0.24300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.390 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 * | 0.286 * |
| Total number of observations | 693751 * | |
| Number of reflections | 93244 | |
| <I/σ(I)> | 5.2 | 2.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 283 | Maita, N., (2001) Acta Crystallogr., D57, 1153. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 24 (%(v/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 75 (mM) | pH7.5 |
| 4 | 1 | reservoir | 50 (mM) | ||
| 5 | 1 | reservoir | phenylalanine | 5 (mM) |
