1IRM
Crystal structure of apo heme oxygenase-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.709 |
| Spacegroup name | P 32 |
| Unit cell lengths | 70.210, 70.210, 140.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.625 - 2.550 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.30700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dve |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.480 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.059 * | 0.250 * |
| Total number of observations | 93875 * | |
| Number of reflections | 25283 | |
| <I/σ(I)> | 9.2 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | ammonium sulfate, PEG400, HEPES, stronthium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 2 | 1 | reservoir | PEG400 | 2 (%) | |
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | HEPES-NaOH | 0.1 (M) | pH8.0 |
| 5 | 1 | drop | protein | 20 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
| 7 | 1 | drop | 50 (mM) |
