1IR3
PHOSPHORYLATED INSULIN RECEPTOR TYROSINE KINASE IN COMPLEX WITH PEPTIDE SUBSTRATE AND ATP ANALOG
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | FUJI |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.505, 66.505, 139.095 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1irk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.816) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.900 | |
| Rmerge | 0.065 | 0.153 * |
| Total number of observations | 93535 * | |
| Number of reflections | 28286 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 98.6 | 90.6 * |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | 22% PEG 8000, 100 MM TRIS-HCL, PH 7.5, 2% ETHYLENE GLYCOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | ethylene glycol | 2 (%) | |
| 2 | 1 | drop | AMP-PNP | 1 (mM) | |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | drop | peptide substrate | 0.25 (mM) | |
| 5 | 1 | drop | PEG8000 | 11 (%) | |
| 6 | 1 | drop | Tris-HCl | 50 (mM) | |
| 7 | 1 | drop | ethylene glycol | 1 (%) | |
| 8 | 1 | reservoir | PEG8000 | 22 (%) | |
| 9 | 1 | reservoir | Tris-HCl | 100 (mM) |
