1ILK
INTERLEUKIN-10 CRYSTAL STRUCTURE REVEALS THE FUNCTIONAL DIMER WITH AN UNEXPECTED TOPOLOGICAL SIMILARITY TO INTERFERON GAMMA
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.274, 70.274, 70.308 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.047 * |
| Data reduction software | MSC |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.061 |
| Number of reflections | 15324 |
| Completeness [%] | 80.8 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 10 (%) | |
| 3 | 1 | drop | sodium cacodylate | 50 (mM) | pH6.5 |
| 4 | 1 | reservoir | ammonium sulfate | 60 (%sat) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
